Table 1. Primer pairs used in PCR experiments.
Gene / Accession number | Gene ID | OMIM number | Primer sequences (5′-3′) | Product (bp) |
---|---|---|---|---|
ACTB |
60 |
102630 |
F: ATGGCCACGGCTGCTTCCAGC |
237 |
NM_001101 |
|
|
R: CATGGTGGTGCCGCCAGACAG |
|
OPN |
6696 |
166490 |
F: GCCGAGGTGATAGTGTGGTT |
178 |
NM_001251830.1 |
|
|
R: CAATCAGAAGGCGCGTTCAG |
|
CD44 |
960 |
107269 |
F: CCGCTATGTCCAGAAAGGA |
195 |
NM_001202557.1 |
|
|
R: CTGTCTGTGCTGTCGGTGAT |
|
NFAT5 |
10725 |
604708 |
F: TCACCATCATCTTCCCACCT |
174 |
NM_006599.3 |
|
|
R: CTGCAATAGTGCATCGCTGT |
|
VEGFA188, 164, 120 |
7422 |
192240 |
F: CCTGGTGGACATCTTCCAGGAGTA |
479; 407; |
NM_003376.5 |
|
|
R: CTCACCGCCTCGGCTTGTCACA |
275 |
NM_001287044.1 |
|
|
|
|
NM_001025370.2 |
|
|
|
|
bFGF |
2247 |
134920 |
F: AGAGCGACCCTCACATCAAG |
234 |
NM_001361665.1 | R: ACTGCCCAGTTCGTTTCAGT |
s, sense. as, anti-sense.