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. 2019 Jan 7;12(1):71–85. doi: 10.1016/j.molp.2018.10.009

Figure 2.

Figure 2

EXPA2 Spatiotemporal Expression Pattern Is Tightly Associated with Endosperm Expansion and Depends on an Embryo Signal(s).

(A)ProEXPA2:GUS seeds were imbibed on half-strength Murashige and Skoog (½ MS) medium for the indicated times, and stained overnight to detect GUS activity after seed coats were removed. GUS staining was visualized after bleaching the testa.

(B)ProEXPA2:GUS seeds were imbibed on ½ MS medium in the absence or presence of ABA (10 μM), PAC (20 μM), or PAC (20 μM) + GA (10 μM) for 24 h, and stained overnight after seed coats were removed.

(C) (C) ProEXPA2:GUS seeds were imbibed on ½ MS medium and, at the indicated times (min), seed coats were separated from embryos, incubated on ½ MS for a total of 24 h and stained overnight.