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. 2019 Jul 4;30(2):753–766. doi: 10.1093/cercor/bhz123

Figure 2.

Figure 2

Activity-dependent regulation of Cx30 expression relies on mGluRs and calcium signaling. (A) Immunoblot detection of Cx30 levels in hippocampal acute slices from wild-type (+/+) or IP3R2−/− mice, treated with either BAPTA (25 μM, 1 h), TTX (0.5 μM, 1 h), or 0Mg-P (100 μM, 3 h). GAPDH was used as a loading control. (B) Quantitative analysis of Cx30 expression. Relative expression levels of Cx30 normalized to GAPDH levels showing that Cx30 expression is reduced in the absence of intracellular free calcium (BAPTA, n = 5), and its activity-dependent regulations by TTX (n = 5) and 0Mg-P (n = 5) are reduced or inhibited, respectively, in hippocampal acute slices from IP3R2−/− mice; control ratio is set to 1. (C) Immunoblot detection of Cx30 expression in hippocampal acute slices incubated in 0Mg-P for 3 h with or without antagonists of mGluRs (LY341495, 20 μM), P2Y1Rs (MRS2179, 10 μM), or GABABRs (CGP55845, 2 μM). (D) Quantitative analysis of Cx30 expression. Relative expression levels of Cx30 normalized to GAPDH levels showing that Cx30 expression in 0Mg-P (n = 7) was decreased to control level by inhibition of mGluRs (LY, n = 4) but was unaltered by blockade of P2Y1Rs (MRS, n = 4) or GABABRs (CGP, n = 4; ANOVA and Dunnett’s post hoc test); control ratio is set to 1 (n = 7). Asterisks indicate statistical significance (*P < 0.05, **P < 0.01).