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. 2019 Jul 4;30(2):753–766. doi: 10.1093/cercor/bhz123

Figure 6.

Figure 6

Neuronal activity drives Cx30 to PAPs. (A) Activity-dependent relocalization of astroglial Cx30 proteins assessed by western blotting of crude synaptosomal membrane fractions. Cx30 protein level in crude synaptosomal fraction was increased in 0Mg-P (100 μM, 3 h, n = 3). Quantitative analysis of Cx30 expression in crude synaptosomal membrane fraction. (B) Images showing colocalization analysis of Cx30 and PSD95 puncta using SIM. Scale bar, 2 μM. The white square indicates the magnified small region of interest. Scale bar, 1 μM. (C) Neuronal activity does not alter the distance of Cx30 puncta within PAPs to synapses. Quantitative analysis of the mean distance of Cx30 puncta to pre- (VGLUT1, control: n = 1107, 0Mg-P: n = 862) and postsynaptic (PSD95, control: n = 1089, 0Mg-P: n = 599) markers. Asterisks indicate statistical significance (**P < 0.01).