Figure 5.
Expression of G6PD is negatively regulated by miR-613. (A) The mRNA expression of G6PD was measured by reverse transcription-quantitative PCR after Eca109 cells were transfected with either miR-NC or miR-613 mimic. (B) Protein level of G6PD was detected by western blotting post-transfection, and densitometric analysis was performed. GAPDH was used for normalization. **P<0.01 vs. controls. miR, microRNA; G6PD, glucose-6-phosphate dehydrogenase.