Figure 5.

PRP inhibits UVB-induced MMP-1 and tyrosinase upregulation. (A) MMP-1, tyrosinase, fibrillin and tropoelastin mRNA levels in human skin grafts after UVB and/or PRP treatments were determined by reverse transcription-PCR using GAPDH as an internal control. Data are presented as the mean ± SD of three independent experiments. (B) MMP-1, tyrosinase, tropoelastin and β-actin protein levels in human skin grafts after UVB and/or PRP treatments were assessed by immunoblotting. Bar plots show the quantified fold changes of the western blotting bands. ^**P<0.01. (C) Distribution of MMP-1, tyrosinase, fibrillin and tropoelastin in human skin grafts after UVB and/or PRP were detected by immunofluorescence staining (magnification, x200). PRP, platelet-rich plasma; MMP-1, matrix metalloproteinase-1; UV, ultraviolet.