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. 2020 Feb 20;11(10):2737–2748. doi: 10.7150/jca.36822

Figure 8.

Figure 8

The expression of miRNA-18a/HIF1a/PVT1 in ccRCC cell lines and clinical sample. (A) The relative expression levels of miR-18a in ccRCC cell lines compared with normal kidney cell lines (HK2); (B) The relative expression of miRNA-18a in 20 paired tissues [log2(Tumor/Normal)]; (C) Immunohistochemistry (IHC) detection of HIF1A in normal and tumor tissues. Scale bar,Top:100μm, bottom:25μm; (D) Ratio of HIF1A positive cells to total cells in the tissues. The number of cells in three different field of view was counted; (E) The HIF1A protein expression levels were screened through Western blotting analysis in 6 paired tissues (N: normal, T: tumor); (F) The relative expression levels of PVT1 in ccRCC cell lines and HK2; (G) The relative expression of PVT1 in 20 paired tissues [log2(Tumor/Normal)].