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. 2020 Mar 4;11(10):3002–3012. doi: 10.7150/jca.40592

Figure 3.

Figure 3

SNHG6 might exerted its biological role in CCA cells via modulating E2F8. mRNA expression profile was analyzed after SNHG6 knockdown in HCCC-9810 cell. (A). KEGG pathway enrichment analysis of the differentially expressed genes presented by Q value. (B). Rich Factor was also used to analyze the differentially expressed genes. The larger the Rich Factor, the greater the degree of enrichment. (C). Flow cytometry assay was used to analyze the cell cycle, and cell cycle was arrested in G0/G1 phase after SNHG6 was knocked down. (D). Heat map of the differential expressed mRNAs involved in cell cycle pathway. (E). qRT-PCR and (F). western blot was used to measure the expression of E2F8 in HCCC-9810 and RBE cell line after transfected with si-SNHG6 or si-NC. (F). qRT-PCR was used to detect the relative expression of E2F8 in CCA tissues (n=22) and adjacent normal tissues (n=14). Data were shown as mean ± SD. * p < 0.05, ** p < 0.01.