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. 2020 Mar 4;11(10):3041–3051. doi: 10.7150/jca.39645

Figure 5.

Figure 5

Experimental validation by gastric cancer cells. (A) Western blot was used to detect the expression level of MSRB3 in different gastric cancer cell lines. (B) MKN45 cell was knockdown of MSRB3 gene and western blot was used to detect the expression level of MSRB3. (C) MKN45 cell was knockdown of MSRB3 gene. MTT assay was used to detect the cell proliferation rates in 0h, 24h, 48h and 72h. Data are means ± SD in three independent experiment (*P < 0.05). (D) Transwell assay was performed to detect the migration of MKN45 cell after silencing MSRB3 for 48h. Data are means ± SD in three independent experiment (****P < 0.0001). (E) Western blot was used to assess the expression levels of phosphor-Akt, Akt, phosohor-Erk, Erk and Actin in MKN45 cells by silencing MSRB3 for 48h.