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Detection of the migration ability of gastric cancer cells in vitro. A, B, C. BGC-823, SGC-7901 and GES-1 cells were co-cultured in the CM from M2 (OE CM or VEC CM). The ratio of normal medium to conditioned medium was 3:1. The cells that migrated through the transwell were stained with crystal violet and counted using ImageJ. The results are shown as the means (±s.d.). D. OE CM-treated gastric cancer cells expressed low levels of p-mTOR, p-AKT, and p-70S6K.
