Figure 3.

miR-34a-5p decreased the LEF1 expression levels in a dose-dependent manner. (a) Left panel showed relative miR-34a-5p expression in Eca109 and TE1 cells after transfection with various concentrations of miR-34a-5p mimic (50, 80, and 100 nM) or miR-ctrl analyzed by qRT-PCR. Right panel showed the corresponding relative LEF1 mRNA expression analyzed by qRT-PCR. (b) Western blotting analysis of LEF1 protein levels after cells were transfected with miR-34a-5p mimics in a dose-dependent manner. (c-e) Relative miR-34a-5p expression in Eca109 and TE1 cells after transfection with miR-34a-5p inhibitor (100nM) or miR-ctrl analyzed by qRT-PCR and western blotting. *P<0.05, **P<0.01