Table I.
Primer sequences.
| Primer | Sequences (5'-3') |
|---|---|
| Wnt3 F | ATCATAAGGGGCCGCCTGGCGAAGGCTGG |
| Wnt3 R | CTTGCAGGTGTGCACGTCGTAGA |
| TCF7 F | CTGCAGACCCCTGACCTCTCT |
| TCF7 R | ATCCTTGATGCTAGGTTCTGGTGT |
| FZD8 F | CTGGTGGAGATCCAGTGCTC |
| FZD8 R | TTGTAGTCCATGCACAGCGT |
| Smo F | ACCTATGCCTGGCACACTTC |
| Smo R | AGGAAGTAGCCTCCCACGAT |
| Gli1 F | CCAGAGTTCAAGAGCCTGG |
| Gli1 R | CCTCGCTCCATAAGGCTCAG |
| Gli2 F | GTTCCAAGGCCTACTCTCGCCTG |
| Gli2 R | 5'-CTTGAGCAGTGGAGCACGGACAT-3' |
| Gli3 F | GGGTGAACAGCATCAAAATGGAG |
| Gli3 R | CCGATAGCCATGTTGGTGG |
| β-catenin F | TCGCCAGGATGATCCCAGC |
| β-catenin R | GCCCATCCATGAGGTCCTG |
| GAPDH F | GACCTGCCGTCTAGAAAAAC |
| GAPDH R | CTGTAGCCAAATTCGTTGTC |
F, forward; FZD8, frizzled class receptor 8; Gli, GLI family zinc finger; R, reverse; Smo, smoothened, frizzled class receptor; TCF, transcription factor.