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. 2020 Feb 26;19(4):3150–3158. doi: 10.3892/etm.2020.8553

Figure 3.

Figure 3

Restoration of T cell function by TPFS-201. (A) PD-L1 expression in A549 cells treated with 100 U/ml interferon-γ (upper). PD-1 expression in Jurkat T cells stimulated with 12.5 ng/ml PMA and 0.25 µg/ml PHA (lower). (B) Decreased apoptosis and (C) enhanced IL-2-luciferase expression in Jurkat T cells co-cultured with TPFS-201-treated A549 cells. A549 cells were pretreated with interferon-γ alone or in combination with TPFS-201 and co-cultured with Jurkat T cells stimulated with 12.5 ng/ml PHA and 0.25 µg/ml PMA. Caspase 3/7 activities and IL-2-directed luciferase expression were determined as indicators of T cell function. Caspase 3/7 activities or luciferase expression in Jurkat T cells co-cultured with TPFS-201-treated A549 cells were normalized to that of T cells co-cultured with vehicle-treated A549 cells, which was arbitrarily set as 100%. Results are presented as the mean ± standard deviation from three independent experiments. *P<0.05 vs. co-cultured vehicle-treated A549 cells. PD-L1, programmed death-ligand 1; PMA, phorbol 12-myristate 13-acetate; PHA, phytohemagglutinin; IL, interleukin.