Figure 4.

Marginal inhibition of PD-L1 expression in a murine cell line by TPFS compounds. (A) The B16F10 mouse melanoma cell line was pre-stimulated with mouse IFN-γ and cultured in the absence or presence of the indicated concentrations of TPFS-202. After 48 h, cells were harvested and PD-L1 was analyzed using flow cytometry. Histograms (upper) and bar graphs of mPD-L1 median fluorescence intensities (lower) represent data acquired from three independent experiments. (B) B16F10 or RAW264.7 cells were stimulated with IFN-γ and cultured in the absence or presence of various concentration of TPFS compounds for 24 h, after which RNA was extracted and used for reverse transcription-quantitative PCR analysis. All samples were normalized to the expression of GAPDH. Data are presented as the fold change of mPD-L1 expression in comound-treated groups relative to the vehicle control. All graphs represent results from three independent experiments. ^*P<0.05 vs. untreated controls. PD-L1, programmed death-ligand 1; IFN, interferon.