Figure 2.

MiR-146a-5p inhibits the proliferation and chemoresistance of PDAC cells. (A) Cell proliferation in PANC-1 and SW1990 cells transfected with miR-146a-5p mimics, inhibitors or their respective negative controls (NCs) was detected by the CCK-8 assay. (B-C) Representative images and statistical analysis of colony formation in PANC-1 and SW1990 cells transfected with miR-146a-5p mimics, inhibitors or their respective NCs (*P < 0.05, **P < 0.01). (D-E) IC₅₀ value of gemcitabine (GEM) measured in PANC-1 and SW1990 cells transfected with miR-146a-5p mimics, inhibitors or their NCs (*P < 0.05, **P < 0.01). (F-I) The apoptosis rate of PANC-1 and SW1990 cells transfected with miR-146a-5p mimics, inhibitors or their NCs and treated with GEM (10 µM, 48 h) was measured by flow cytometric analysis using annexin V/7-AAD staining (*P < 0.05, **P < 0.01).