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. 2020 Mar 4;10(9):3925–3938. doi: 10.7150/thno.41378

Figure 3.

Figure 3

IL-12 and IL-23 affect bone formation and resorption in vivo. (A) The expression levels of osteoblast-related genes (ALP and OCN) in cranium from WT, IL-12p35-/-, and IL-12p40-/- mice. n = 8 per group. (B) Serum levels of OCN. n = 8 per group. (C) ALP staining of femur sections from WT, IL-12p35-/-, and IL-12p40-/- mice. n = 4-5 per group. Scale bar, 20 µm. (D) Quantification of ALP activity was shown via images from the groups described in C. (E) The expression levels of osteoclast-specific genes (TRAP and RANKL) in calvarial bone from WT, IL-12p35-/-, and IL-12p40-/- mice. n = 6 per group. (F) Serum levels of CTX-1. n = 6 per group. (G) TRAP staining of femur sections from WT, IL-12p35-/-, and IL-12p40-/- mice. n = 4-5 per group. Scale bar, 20 µm. (H) Quantification of TRAP activity was shown via images from the groups described in G. ALP, alkaline phosphatase; CTX-1, collagen type I cross-linked C-telopeptide; OCN, osteocalcin; RANKL, receptor activator of NF-κB ligand; TRAP, tartrate-resistant acid phosphatase; WT, wild-type. Results are shown as mean ± S.D. *p<0.05, **p<0.01, ***p<0.001.