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. 2020 Mar 4;10(9):3980–3993. doi: 10.7150/thno.41028

Figure 4.

Figure 4

Autophagosome accumulation induced by AngII was potentiated by TMEM16A downregulation. (A) Cells were treated with TMEM16A siRNA (TM siRNA, 25 nmol/L) or scrambled siRNA (scr.RNA) for 48 h before AngII stimulation. The protein expression of LC3B-II, Beclin-1 and p62 were measured. **P < 0.01 vs. control; ##P < 0.01 vs. AngII, one-way ANOVA. n = 6. (B) MASMCs were transfected with TM siRNA and then treated with CQ and AngII for 24 h, after which LC3B-II expression was measured. **P < 0.01 vs. control; ##P < 0.01 vs. AngII; &&P < 0.01 vs. AngII + CQ, one-way ANOVA. n = 6. (C) mRFP-GFP-LC3 expressing cells were treated with scr.RNA or TM siRNA, with or without AngII stimulation. Images were acquired with a confocal microscope. Scale bars, 10 µm. (D and E) Autophagosome (GFP+/RFP+) (D) and autolysosome (GFP-/RFP+) (E) numbers were calculated, using 100 cells from each experiment and averaged. **P < 0.01 vs. control; ##P < 0.01 vs. AngII, one-way ANOVA. n = 4.