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Cetuximab-800CW binding specificity. Cetuximab-800CW fluorescence was located around the tumor cells, indicating specific binding. A Hoechst-DAPI staining was performed to visualize cell nuclei and the FITC channel was used to discriminate between autofluorescence and cetuximab-800CW derived fluorescence on the 800 nm channel. Abbreviations: H/E: Hematoxylin and Eosin. FITC: Fluorescein isothiocyanate.
