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. 2020 Mar 4;10(9):3880–3891. doi: 10.7150/thno.41383

Figure 2.

Figure 2

PHB is bound directly to Rab27a in Melan-a cells. A IB analysis for Rab27a, PHB, Mlph, and Myosin-Va of Mlph-KD Melan-a cells were treated with Mlph of siRNAs (20 nM) for 72h. B Anti-Rab27a and PHB immunoprecipitated (IP) cells. As indicated, were performed for O/N at 4 °C followed by western blot analysis to check co-immunoprecipitated (co-IP) proteins (WB). Whole cell lysates were analyzed for total protein levels. C Representative cell images compared Leaden Melan-a cells with control, Melan-a cells. D IB analysis for Rab27a, PHB, Mlph, Myosin-Va of Leaden Melan-a cells. E Anti-Rab27a, PHB, and Myosin-Va IP were performed for O/N at 4 °C followed by western blot analysis to check co-IP proteins (WB). Whole cell lysates were analyzed for total protein levels.