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PHB is bound to GTP and GDP-bound form of Rab27a. A IB analysis for Rab27a, PHB, Mlph, and Rab3GEP (MADD) of Rab3GEP-KD Melan-a cells. As indicated, cells were treated with Rab3GEP of siRNAs (50nM) for 72h. B Anti-Rab27a, PHB, and Mlph immunoprecipitated (IP) were performed for O/N at 4 °C followed by western blot analysis to check co-immunoprecipitated proteins (WB). Whole cell lysates were analyzed for total protein levels.
