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PHB is bound directly to Mlph in Melan-a cells. A IB analysis for Rab27a, PHB, Mlph, and myosin-Va of Ashen Melan-a cells. B COS7 monkey kidney cells were transfected with PHB and Mlph plasmids. Anti-histidine immunoprecipitated (IP) were performed for 3 h at 4 °C followed by western blot analysis to check co-IP (WB). Whole cell lysates were analyzed for total protein levels.
