Figure 5.

PHB is bound directly to Rab27a and Mlph, not myosin-Va. A, B, C PLA assay was performed with Melan-a cells stained with DAPI (blue) for nuclei and PLA signals (red) indicated protein interaction events (scale bar=100 µm). A Representative images of the PHB-Rab27a complex. For a negative control, the cells were stained with anti-Rab27a or PHB IgG antibodies, only. B Representative images of PHB-Mlph complex. For negative controls, the cells were stained with anti-Mlph or PHB-immunoglobin G (IgG) antibodies, only. C Representative images indicating an absence of PHB-Myosin Va interaction. For negative controls, the cells were stained with anti-Myosin-Va or PHB-IgG antibodies, only. D Quantification of PLA data (mean ± standard deviation, n = 3).