Figure 6.

Rab27a and Mlph are bound to specific domains of PHB. A Alignment of PHB sequences (mouse) that PHB domains present for a transmembrane domain, a prohibitin domain, and a coiled-coil domain. B, C Anti-histidine pull-down immunoprecipitation was performed with Mlph-transfected-(B) and Rab27a transfected-(C) cells for 1 h at 4 °C followed by western blot analysis to check immunoprecipitated proteins (WB). Rab27a transfected Melan-a cells were treated with proteasome inhibitor MG132 (1 µM) for 3 h before cell collection.