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. 2020 Mar 4;10(9):4088–4100. doi: 10.7150/thno.42416

Figure 5.

Figure 5

IL-37 increased GEM efficacy and inhibited PDAC cells migration in vitro. Flow cytometry to analyze the G1 phase distribution (A) and apoptosis (B) of PANC-1, MIA-PaCa2 and SW1990 cell lines treated with rhIL-37 (100 ng/ml) and Gem (2 µM) for 24 h. (C) Left, wound-healing assays comparing the motility of PANC-1, MIA-PaCa2 and SW1990 cell lines treated with rhIL-37 protein for 24 h and 36 h. Right, comparison of the migration of PANC-1, MIA-PaCa2 and SW1990 cell lines treated with rhIL-37 protein for 18 h. (D) Western blot analysis of IL-37 expression in SW1990 and MIA-PaCa2 cell lines transfected with pLV-IL37 plasmid (2 µg). (E) The secretion of IL-37 in culture supernatant with transfected cells examined by ELISA kit. Wound-healing (F) and transwell (G) analysis of migration in PDAC cell lines transfected with pLV-IL37 plasmid. The data are presented as the means ± SD from three independent experiments. *P < 0.05 and **P < 0.01.