Table I. Standard and real-time PCR results for α1,3GT, Prkdcscid, and IL-2Rγtm1Wjl Genes.
| Gene | Allele | C57BL/6 GTKO | NSG Parental | NSG/α-Galnull |
|---|---|---|---|---|
| α1,3GT | NeoR | ++a | −b | ++ |
| Wild-type | − | ++ | − | |
| Prkdcscid | Mutant | − | 25.4 | 25.0 |
| Wild-type | 29.3 | − | − | |
| IL-2Rγtm1Wjl | Mutant | − | 26.8 | 26.6 |
| Wild-type | 27.7 | − | − |
Genomic DNA was isolated from each mouse. Samples were analyzed by standard PCR or real-time PCR for wild-type or mutant alleles. Either the cycle threshold or the amplification efficiency for each reaction is shown.
a
Samples with detectible PCR amplification of the correct size are shown with “++” sign.
b
Samples with undetectable signal are shown with a “−” sign.