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. 2020 Mar 6;204(7):1998–2005. doi: 10.4049/jimmunol.1901385

Table II. PCR and real-time PCR primers and probes.

Name Sequence
α1,3GT gene
 GT-NeoR (forward) 5′-CTATCGCCTTCTTGACGAGTTCTTCTGAGG-3′
 GT wild type (reverse) 5′-CTTGAAAGACTTGATCCACGTCCATGCAG-3′
 GT wild type (forward) 5′-CGTGCACCTGAACCCTCTACATTCCTTAC-3′
Prkdcscid
 Forward 5′-CAGACAATGCTGAGAAAAGGAG-3′
 Reverse 5′-CTGCATTCACAAGTCTTACCAAG-3′
 Mutant probe 5′-[6FAM]TAAAATACGCTAAGCTAAGAGAAAG[BHQ1]-3′
 Wild-type probe 5′-[HEX]TAAAATACGCTATGCTAAGAGAAAG[BHQ1]-3′
IL2Rγ
 Forward 5′-AAGAGATTACTTCTGGCTGTCAG-3′
 Mutant reverse 5′-ATGCTCCAGACTGCCTTG-3′
 Mutant probe 5′-[6FAM]AAAGCGCCTCCCCTACCCG[BHQ1]-3′
 Wild-type reverse 5′-CTCTGGGGTTTCTGGGG-3′
 Wild-type probe 5′-[HEX]TGGAGCTGGACAACAAATGTCTGGT[BHQ1]-3′