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. 2020 Jan 7;31(3):656–662. doi: 10.1021/acs.bioconjchem.9b00804

Figure 1.

Figure 1

Development of bioluminescent antibodies using Gx-NL fusion proteins. (A) Gx-NL fusions contain a pBPA moiety (inset), which is able to conjugate to the backbone of the Fc domain of an antibody under 365 nm illumination. (B) Schematic representation of protein sequence of Gx-NL fusion proteins. (C) Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) analysis of purified adapter proteins. (D) Normalized luminescence spectra of different colored adapter proteins. (E) Luminescence spectra of 100 pM fusion proteins with multiple tandem repeats of NanoLuc.