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Photoconjugation of Gx-NL fusion proteins to cetuximab. (A) Nonreducing SDS-PAGE analysis of photo-cross-linking 8 equiv of Gx-mNG-NL (3.2 μM) to cetuximab (0.4 μM) with increasing illumination times. (B) Nonreducing SDS-PAGE analysis of 1 h photoconjugation of 0.4 μM cetuximab with 3.2 μM of various protein G–luciferase fusion proteins.
