Skip to main content
. 2020 Jan 7;31(3):656–662. doi: 10.1021/acs.bioconjchem.9b00804

Figure 3.

Figure 3

Immunostaining of cell surface receptors on A431 and SK-BR-3 cells using bioluminescent antibodies. Flow cytometry of A431 cells stained with 10 nM cetuximab (C) or trastuzumab (T) photo-cross-linked with (A) Gx-mNG-NL (mNG) or (B) Gx-NL-Cy3 (Cy3). (C, D) Plate reader read-out of stained cells (A, A431; S, SK-BR-3) with 1 nM bioluminescent antibody (C, cetuximab; T, trastuzumab) with either (C) luminescence or (D) fluorescence detected. (E) Analysis of the experiments described in (C) and (D) using a digital camera.