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ELISA assay for the detection of the anti-cetuximab antibody using bioluminescently labeled cetuximab. A 1 μg/mL cetuximab-coated plate was incubated with anti-cetuximab antibody (1.2–10 000 ng/μL) and subsequently with 1.33 nM Gx-mNG-NL–cetuximab antibody. Both fluorescence and luminescence were recorded using a plate reader.
