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Bioluminescent antibody used as detection antibody in immunostaining of a Western blot. (A) Reducing SDS-PAGE gel showing the purified 65 kDa 2·HA-tag protein. (B) Coomassie-stained SDS-PAGE gel of cell lysate spiked with various dilutions (1–3 125×) of the 2·HA-tag protein. (C, D) Western blot of (B) stained with 3.32 nM Gx-NL-anti-HA (C) or Gx-NL3-anti-HA (D).
