Table 1.
Gene | Forward primer (5′-3′) | Reverse primer (5′-3′) |
---|---|---|
miR-338-5p | GGGAACAATATCCTGGTGC | GTGCAGGGTCCGAGGT |
Shh | AGCTGGAGAAGTTTAGGGTG | CAAGCCAGGGCAGAGGTAG |
Ihh | CCGCAATAAGTATGGACTGC | TTGGCTGCGGCCGAGTGCT |
Dhh | AGGAGCGGGTGAACGCTTTG | GCGCCAGCAACCCATACTTG |
E-cadherin | CCTCGACACCCGATTCAAAG | CCACTGTATTCAGCGTGAC |
MMP-2 | TGCACTGATACCGGCCGCAG | AACTTGCAGGGCTGTCCTTC |
GAPDH | GTCTCCTCTGACTTCAA | ACCACCCTGTTGCTGTA |
Note: Shh—Sonic Hedgehog; Ihh—Indian Hedgehog; Dhh—Desert Hedgehog; MMP-2—matrix metalloproteinase-2.