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. 2007 Jan 31;1(6):3088–3093. doi: 10.1038/nprot.2006.452

Table 3.

Plasmid yields using this protocol.

Batcha OD 260/280 Plasmid (mg l−1)b
1 1.91 110
2 1.84 120
3 1.81 95

aThe host E. coli strain used was TOP10 and 17 l of culture was processed after the OD 600 nm reached a plateau around 30.

bThe yield of plasmid pcD-VP1 was determined after PEG 8000 purification.