Fig. 5.

BCR stimulation of PLCγ2 KO DT40 cells transfected with PLCγ2 constructs. a PLCγ2 KO DT40 cells were transiently transfected with empty plasmid, normal, and mutant PLCγ2^M1141K construct corresponding to our patients’ genetic variant. After overnight incubation, the transfected DT40 cells were incubated in Ca²⁺-free media and stimulated via BCR followed by addition of exogenous Ca²⁺. Each data point is representative of three independent experiments with measurements shown in 15-s intervals. Viable cells were analyzed for 60 s prior to BCR stimulation in order to establish an average baseline by which the relative fold increase in fluorescence is measured. b Western blot showing equal transfection expression of PLCγ2 constructs