Table 1.
Primers used to amplify and sequence the entire E1A, penton base, hexon, and fiber genes of HAdV-B [P14H11F14] strain SD77001
| Primer | Sequence (5’ - 3’) | Size (bp) |
|---|---|---|
| E1A_1f | TGCTATGAAGACGGGTTTCC | 748 |
| E1A_1r | CGTCCGAAGCGTTCTCTAAC | |
| E1A_2f | ATGGCAGGGTGGAGTATTTG | 700 |
| E1A_2r | AACACTGGCAGCCTTCACTC | |
| Penton_1f | TCGCTTGGGTGGTATGTTGTA | 751 |
| Penton_1r | CACCAATGTCACTTTCAAGCA | |
| Penton_2f | GTGATGGTGTCCAGAAAACCT | 807 |
| Penton_2r | ATCTGAGGTGGTGAGCAATGT | |
| Penton_3f | GCTAACGCTGGAGAGGTCAG | 790 |
| Penton_3r | CACGGGATGTTGGGTAGAAC | |
| Hexon_1f | TCACAGCAGCAGAGGAAAAA | 701 |
| Hexon_1r | TTTCGGTCTTTCCATCAAGG | |
| Hexon_2f | TCAGTGGATTGCAGAAGGTG | 996 |
| Hexon_2r | CTTCGCCATAGATTGGCTTG | |
| Hexon_3f | ATGCAGTGGTTGACTTGCAG | 907 |
| Hexon_3r | TTGGCAGGAATGGGATAGAG | |
| Hexon_4f | CAAAAACCTGCTGCTTCTCC | 908 |
| Hexon_4r | GCCACATGGTTCTGTCACAC | |
| Hexon_5f | CAGATGCTCGCCAACTACAA | 845 |
| Hexon_5r | TCATCCGAGAATCCAAAAGG | |
| Fiber_1f | TTAACCCCGCTAACAACCAC | 899 |
| Fiber_1r | GAAGGGGGAGGCAAAATAAC | |
| Fiber_2f | GGCCAAAGAGCTCAGAGATG | 804 |
| Fiber_2r | ATCCGGCTCCTAGGGATAAA |
Primers were designed according to human adenovirus 55 strain QS-DLL (GenBank accession: FJ643676)