Fig. 6.

BVDV infection in autophagy-knockdown MDBK cells induces enhanced apoptosis. (a) Cell viability. shBCN1-MDBK cells and control-MDBK cells were infected with HJ-1 or NY-1 at an m.o.i. of 5 or 2.5 for 72 h, and MTS was added to the medium to detect the level of absorption. (b, c) Cell apoptosis after treatment with HJ-1 or NY-1 for 48, 72, and 96 h. (d) Apoptosis (%) of shBCN1-MDBK cells and control-MDBK cells after treatment with HJ-1 or NY-1 for 96 h. Significance was analysed using a two-tailed Student’s t-test. *, P < 0.05; **, P < 0.01; ***, P < 0.01