Fig. 7.

BVDV infection upregulates IFN signalling molecules in Beclin 1-knockdown MDBK cells. Intracellular mRNA was extracted from (a) HJ-1-infected shBCN1-MDBK cells or (b) NY-1-infected shBCN1-MDBK cells. Intracellular mRNA expression of IFN-β, OAS-1, IFN-α, and Mx1 was detected by real-time, RT-PCR. GAPDH was used as an internal control. The fold changes in mRNA levels are presented after normalization with a mock-infected control. The data are reported as the mean ± SD (n = 3). Significance was analysed using a two-tailed Student’s t-test. **, P < 0.01; ***, P < 0.001