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Effect of RpSP-A on Marc 145 cell penetration. Cells were incubated for 24 h after treatment with PRRSV alone or after addition of RpSP-A at 1-h intervals in the presence or absence of calcium ions, and then virus titer was measured. Data are presented as mean ± S.D. of three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001 compared to the control
