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Specificity of multiplex PCR for the detection of positive and negative controls using mixed primers. Lanes 1-13: 1, FPV; 2, FBoV; 3, FeAstV; 4, FPV and FBoV; 5, FPV and FeAstV; 6, FBoV and FeAstV; 7, FeAstV, FBoV and FPV; 8, FCV; 9: FHV-1; 10, FCoV; 11, FeLV; 12, FIV; 13, negative control; M, DL2000 DNA marker
