Table 1.

Nucleotide sequences of the primers used for the multiplex PCR assay

Target virus	Target gene	Primer name	Nucleotide sequence (5’-3’)	Amplicon size (bp)
FPV	VP2	FPV-F	CATACATGGCAAACAAATAGAGCA	237
		FPV-R	TGTTTTAAATGGCCCTTGTGTAGA
FBoV	NP1	FBoV-F	AGAACCRCCRATCACARTCCACT	465
		FBoV-R	TGGCRACCGCYAGCATTTCA
FeAstV	RdRp	FeAstV-F	GCGGATTGGGCATGGTTTAGA	645
		FeAstV-R	ACCCCTCGTTTGGATCGTTACCT