Fig. 2.

Antigenicity of spike (S) protein fragments of turkey coronavirus (TCoV). IFA results with untransfected COS-7 cells as a negative control or cells transfected with various plasmids encoding fragments covering the whole S protein of TCoV are shown. The positive IFA result obtained with mouse anti-His monoclonal antibodies (Anti-His MAbs) indicates expression of recombinant protein fused with a His-tag from the transfected plasmid in COS-7 cells. The positive IFA result obtained with anti-TCoV serum indicates that the expressed S fragment from the transfected plasmid contained antigenic epitopes. (a) Summary of IFA results for anti-His MAbs or anti-TCoV serum reacting with COS-7 cells transfected with various plasmids encoding TCoV S fragments. (b) Photomicrography of untransfected COS-7 cells as negative acontrol or cells transfected with various plasmids encoding TCoV S fragments in an IFA with anti-His MAbs or anti-TCoV serum. Magnification, 400x