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. 2011 May 19;156(9):1525–1535. doi: 10.1007/s00705-011-1020-1

Table 2.

Characterization of polyclonal antibodies induced by the TCoV S fragments S1, 4F/4R, Mod4F/Epi4R, and 6F/6R

(a)
Serum used in IFA COS-7 COS-7 with pTriEx3-S1
 Normal serum Negative Negative
 Anti-TCoV serum Negative 1:40 – Positive
 Anti-DNA pTriEx3-4F/4R Negative 1:20 – Positive
 Anti-DNA pTriEx3-4F/4R & 4F/4R S protein Negative 1:40 – Positive
 Anti-DNA pTriEx3-S1 Negative 1:40 – Positive
(b)
Serum Dilution
Trial 1 1280 2560 5120 10240
 Anti-TCoV serum + + +
40 80 160 320 640 1280
 Anti-DNA pTriEx3-4F/4R & 4F/4R protein + + + + +
 Anti-DNA pTriEx3-4F/4R + +
Anti-DNA pTriEx3-S1 + + +
Trial 2 1280 2560 5120 10240
 Anti-TCoV serum + + +
40 80 160 320 640 1280
 Anti-DNA pTriEx3-4F/4R & 4F/4R protein + + + + +
 Anti-DNA pTriEx3-6F/6R & 6F/6R protein + + + + +
 Anti-DNA pcDNA3.1-Mod4F/Epi4R + + + + +
Trial 3 50 100 200 400 800 1600
 Anti-TCoV serum + + + + +
 Anti-DNA pTriEx3-4F/4R & 4F/4R protein + + + +
 Anti-DNA pTriEx3-6F/6R & 6F/6R protein + + +
 Anti-DNA pcDNA3.1-Mod4F/Epi4R & Mod4F/Epi4R protein + + +