Table 2.
(a) | ||||||
---|---|---|---|---|---|---|
Serum used in IFA | COS-7 | COS-7 with pTriEx3-S1 | ||||
Normal serum | Negative | Negative | ||||
Anti-TCoV serum | Negative | 1:40 – Positive | ||||
Anti-DNA pTriEx3-4F/4R | Negative | 1:20 – Positive | ||||
Anti-DNA pTriEx3-4F/4R & 4F/4R S protein | Negative | 1:40 – Positive | ||||
Anti-DNA pTriEx3-S1 | Negative | 1:40 – Positive |
(b) | ||||||
---|---|---|---|---|---|---|
Serum | Dilution | |||||
Trial 1 | 1280 | 2560 | 5120 | 10240 | ||
Anti-TCoV serum | + | + | + | − | ||
40 | 80 | 160 | 320 | 640 | 1280 | |
Anti-DNA pTriEx3-4F/4R & 4F/4R protein | + | + | + | + | + | − |
Anti-DNA pTriEx3-4F/4R | + | + | − | − | − | − |
Anti-DNA pTriEx3-S1 | + | + | + | − | − | − |
Trial 2 | 1280 | 2560 | 5120 | 10240 | ||
Anti-TCoV serum | + | + | + | − | ||
40 | 80 | 160 | 320 | 640 | 1280 | |
Anti-DNA pTriEx3-4F/4R & 4F/4R protein | + | + | + | + | + | − |
Anti-DNA pTriEx3-6F/6R & 6F/6R protein | + | + | + | + | + | − |
Anti-DNA pcDNA3.1-Mod4F/Epi4R | + | + | + | + | + | − |
Trial 3 | 50 | 100 | 200 | 400 | 800 | 1600 |
Anti-TCoV serum | + | + | + | + | + | − |
Anti-DNA pTriEx3-4F/4R & 4F/4R protein | + | + | + | + | − | − |
Anti-DNA pTriEx3-6F/6R & 6F/6R protein | + | + | + | − | − | − |
Anti-DNA pcDNA3.1-Mod4F/Epi4R & Mod4F/Epi4R protein | + | + | + | − | − | − |