Table 3.
Neutralization activities of polyclonal antibodies raised against S1, 4F/4R, Mod4F/Epi4R, and 6F/6R S fragments
| Serum | VN titera | Log4 (VN titer) | Ratio of log4 VN titerb |
|---|---|---|---|
| Trial 1 | |||
| Anti-TCoV serum | 16 | 2 | 1 |
| Anti-DNA pTriEx3-4F/4R & 4F/4R protein | 2 | 0.5 | 0.25 |
| Anti-4F/4R protein | 3.09 | 0.81 | 0.41 |
| Anti-DNA pTriEx3-S1 | 1.7 | 0.38 | 0.19 |
| Trial 2 | |||
| Anti-TCoV serum | 9.44 | 1.62 | 1 |
| Anti-DNA pTriEx3-4F/4R & 4F/4R protein | 8 | 1.5 | 0.93 |
| Anti-DNA pcDNA3.1-Mod4F/Epi4R | 6.2 | 1.32 | 0.81 |
| Anti-DNA pTriEx3-6F/6R & 6F/6R protein | <1 | 0 | 0 |
| Normal serum | <1 | 0 | 0 |
| Trial 3 | |||
| Anti-TCoV serum | 32 | 2.5 | 1 |
| Anti-DNA pTriEx3-4F/4R & 4F/4R protein | 8 | 1.5 | 0.6 |
| Anti-DNA pcDNA3.1-Mod4F/Epi4R | 7.14 | 1.42 | 0.57 |
| Anti-pcDNA3.1-Mod4F/Epi4R & Mod4F/Epi4R protein | 11.43 | 1.76 | 0.7 |
| Anti-DNA pTriEx3-6F/6R & 6F/6R protein | <1 | 0 | 0 |
| Normal serum | <1 | 0 | 0 |
aTheVN titer is the highest dilution of antiserum that can neutralize TCoV and inhibit the infection of TCoV in 50% of 22-day-old embryonated turkey eggs
bThe ratio of log4 VN titer is between the log4 value of the VN titer in the tested serum and the log4 value of the VN titer in the assay control serum, anti-TCoV serum, used in each assay