Fig. 1.

Evaluation of different siRNAs targeting the RV-G gene using a plasmid co-transfection approach in HEK-293 cells. HEK-293 cells were co-transfected with different RV-G gene silencing constructs expressing different siRNAs (si-G1 to 9) or control siRNA (si-C) along with a target gene reporter plasmid (pDsRed-G) at a 1:2 ratio. At 48 h post-transfection, cells were analysed for red fluorescence. A reduction in red fluorescence compared with the control indicated a reduction in expression of the RV-G-RFP protein. Magnification, 100X. “*” indicates siRNAs (psi-G1, G2 and G7) with marked reduction in expression of RV-G-RFP protein