Fig. 2.

Evaluation of single and dual gene silencing constructs expressing siRNAs targeting the RV-G and N genes to inhibit expression of the RV-G or N genes, using a plasmid co-transfection approach in HEK-293 cells. HEK-293 cells were co-transfected at a 1:2 ratio with dual (psi-G7N) or single (psi-G7 or psi-N) gene silencing construct expressing siRNAs or control siRNA (psi-C) along with target gene reporter plasmids (pDsRed-G or pDsRed-N) expressing RV-G or N with C-terminal fusion with red fluorescent protein (RFP). At 48 h post-transfection, cells were analysed for red fluorescence. A reduction in red fluorescence compared with the control indicated reduction in expression of RV-G-RFP or RV-N-RFP protein. Magnification, 100X