Fig. 3.

Evaluation of single and dual gene silencing constructs expressing siRNAs targeting RV-G and N genes to inhibit RV multiplication in BHK-21 cells. Cells were transfected with dual (psi-G7N) or single (psi-G7 or psi-N) gene silencing construct expressing siRNAs or control (psi-C) siRNA and challenged with the RV-PV-11 strain at an MOI of 0.01. At 48 h post-challenge, the presence of RV in infected BHK-21 cells was detected by staining with FITC-labeled antibody against rabies virus nucleocapsid and counterstained with DAPI. Magnification, 200X