Fig. 4.

Quantitative evaluation of single and dual gene silencing constructs expressing siRNAs targeting the RV-G and N genes to inhibit RV release in BHK-21 cells. The cell culture supernatant was harvested from BHK-21 cells transfected with single and dual gene silencing constructs or a control siRNA construct and challenged with the RV PV-11 strain at an MOI of 0.01. The amount of RV in the supernatant was quantified using rabies fluorescent focus unit (ffu) assay. The percent reduction in RV titer was determined in comparison with control