Table 3.

Schematic diagram showing annealed sense and antisense oligonucleotides along with restriction enzyme sites used for cloning into the shRNA expression vector. The siRNA sequences are underlined

Name	BamHI	Anti-sense strand	Loop	Sense strand	RNA PolIII terminator	XhoI
shRNA-G1	5′-GATCCCAAACTTACTGACTTGGTGGTC TTGATATCCGGACCACCAAGTCAGTAAGTTTTTTTTTCCAA C-3′    3′-GGTTTGAATGACTGAACCACCAGAACTATAGGCCTGGTGGTTCAGTCATTCAAAAAAAAAGGTTGAGCT-5′
shRNA-G2	5′-GATCCCTATTCCTGAGCACTTTCCG TTGATATCCGCGGAAAGTGCTCAGGAATATTTTTTCCAAC-3′    3′-GGATAAGGACTCGTGAAAGGCAACTATAGGCGCCTTTCACGAGTCCTTATAAAAAAGGTTGAGCT-5′
shRNA-G3	5′-GATCCCGCTCCTTTTAGAGACTTATAC TTGATATCCGGTATAAGTCTCTAAAAGGAGCTTTTTTCCAAC-3′    3′-GGCGAGGAAAATCTCTGAATATGAACTATAGGCCATATTCAGAGATTTTCCTCGAAAAAAGGTTGAGCT-5′
shRNA-G4	5′-GATCCCACTTTCAAACACCCTTTTGAG TTGATATCCGCTCAAAAGGGTGTTTGAAAGT TTTTTTCCAAC-3′    3′-GGTGAAAGTTTGTGGGAAAACTCAACTATAGGCGAGTTTTCCCACAAACTTTCAAAAAAAGGTTGAGCT-5′
shRNA-G5	5′-GATCCCGCTTATATGATTCCCATGAAGG TTGATATCCGCCTTCATGGGAATCATATAAGTTTTTTCCAAC-3′    3′-GGCGAATATACTAAGGGTACTTCCAACTATAGGCGGAAGTACCCTTAGTATATTCAAAAAAGGTTGAGCT-5′
shRNA-G6	5′-GATCCCGTTAGAGACTTATACAGGCCTC TTGATATCCGGAGGCCTGTATAAGTCTCTAA TTTTTTCCAAC-3′    3′-GGCAATCTCTGAATATGTCCGGAGAACTATAGGCCTCCGGACATATTCAGAGATTAAAAAAGGTTGAGCT-5′
shRNA-G7	5′-GATCCCACGTCTGAAACTTACTGACTT TTGATATCCGAAGTCAGTAAGTTTCAGACGTTTTTTTCCAAC-3′    3′-GGTGCAGACTTTGAATGACTGAAAACTATAGGCTTCAGTCATTCAAAGTCTGCAAAAAAAGGTTGAGCT-5′
shRNA-G8	5′-GATCCCGTAAACCATTGAAAAACACC TTGATATCCGGGTGTTTTTCAATGGTTTATTTTTTCCAAC-3′    3′-GGCATTTGGTAACTTTTTGTGGAACTATAGGCCCACAAAAAGTTACCAAATAAAAAAGGTTGAGCT-5′
shRNA-G9	5′-GATCCCATTTGGACAGCTGAGATGG TTGATATCCGCCATCTCAGCTGTCCAAATTTTTTTCCAAC-3′    3′-GGTAAACCTGTCGACTCTACCAACTATAGGCGGTAGAGTCGACAGGTTTAAAAAAAGGTTGAGCT-5′
shRNA-N	5′-GATCCCGTAAAGATGCATGTTCAGAGAC TTGATATCCGGTCTCTGAACATGCATCTTTATTTTTTCCAAC-3′    3′-GGCATTTCTACGTACAAGTCTCTGAACTATAGGCCAGAGACTTGTACGTAGAAATAAAAAAGGTTGAGCT-5′
shRNA-C	5′-GATCCCCGTTAAACTGACTCAAGCGCC TTGATATCCGGGCGCTTGAGTCAGTTTAACGTTTTTTCCAAC-3′    3′-GGGCAATTTGACTGAGTTCGCGGAACTATAGGCCCGCGAACTCAGTCAAATTGCAAAAAAGGTTGAACT-5′