Table 1.

Primers used and their annealing temperatures

Virus	Primer sequence (5′-3′)	Amplicon size (bp)	Target gene	Annealing temperature/time	References
BRV	F: GGCTTTWAAACGAAGTCTTC	928	Group (A)–VP6	52 °C/45 s	[29]
	R: GGYGTCATATTYGGTGG				[30]
BRV	F: ATGTATGGTATTGAATATACCAC	881	VP7	52 °C/1 min	[31]
	R: AACTTGCCACCATTTTTTCC
BRV	F: TAT GCT CCA GTN AAT TGG	633	VP4	50 °C/1 min	[32]
	R: ATT GCA TTT CTT TCC ATA ATG
BAstV	F: GAYTGGACBCGHTWTGATGG	432	RdRp	48 °C/1 min	[33]
	R: KYTTRACCCACATNCCAA
BNoV	CBECu-F: AGTTAYTTTTCCTTYTAYGGBGA	532	RdRP	51 °C/45 s	[34]
	CBECu-R: AGTGTCTCTGTCAGTCATCTTCAT
BCoV	F: CCGATCAGTCCGACCAATC	460	N gene	55 °C/45 s	[35]
	R: TAGTCGGAATAGCCTCATCGC
BToV	F: TTCTTACTACACTTTTTGGA	602	Matrix gene	45 °C/45 s	[36]
	R: ACTCAAACTTAACACTAG AC
BVDV	F: ACAAACATGGTTGGTGCAACTGGT	820	Polyprotein	54 °C/ 45 s	[37]
	R: CAGACATATTTGCCTAGGTTCCA

BRV bovine rotavirus, BAstV bovine astrovirus, BNoV bovine norovirus, BCoV bovine coronavirus, BToV bovine torovirus, BVDV bovine viral diarrhea virus, F forward primer, R reverse primer