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. 1980;66(3):215–226. doi: 10.1007/BF01314735

Astrovirus-like, coronavirus-like, and parvovirus-like particles detected in the diarrheal stools of beagle pups

F P Williams Jr 1
PMCID: PMC7086831  PMID: 6778459

Summary

Astrovirus-like, coronavirus-like, and parvovirus-like particles were detected through electron microscopic (EM) examination of loose and diarrheal stools from a litter of beagle pups. Banding patterns obtained from equilibrium centrifugations in CsCl supported the EM identification. Densities associated with the identified particles were: 1.34 g/ml for astrovirus, 1.39 g/ml for “full” parvovirus, and 1.24–1.26 g/ml for “typical” coronavirus. Convalescent sera from the pups aggregated these three particle types as observed by immunoelectron microscopy (IEM). Only coronavirus-like particles were later detected in formed stools from these same pups. Coronavirus and parvo-like viruses are recognized agents of canine viral enteritis, however, astrovirus has not been previously reported in dogs.

Keywords: Electron Microscopic, Infectious Disease, Banding Pattern, CsCl, Particle Type

Footnotes

With 5 Figures

References

  • 1.Appel M. J. G., Cooper B. J., Greisen H., Carmichael L. E. Status report: canine viral enteritis. J. Amer. vet. med. Ass. 1978;173:1516–1518. [Google Scholar]
  • 2.Ashley C. R., Caul E. O., Paver W. K. Astrovirus-associated gastroenteritis in children. J. clin. Pathol. 1978;31:939–943. doi: 10.1136/jcp.31.10.939. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 3.Bachmann P. A., Hoggan M. D., Kurstak E., Melnick J. L., Pereira H. G., Tattersall P., Vago C. Parvoviridae: Second report. Intervirology. 1979;11:248. doi: 10.1159/000149041. [DOI] [PubMed] [Google Scholar]
  • 4.Binn L. N., Lazar E. C., Eddy G. A., Kajima M. Recovery and characterization of a minute virus of canines. Infect. Immun. 1970;1:503–508. doi: 10.1128/iai.1.5.503-508.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5.Binn L. N., Lazar E. C., Keenan K. P., Huxsoll D. L., Marchwicki R. H., Strano A. J. Recovery and characterization of a coronavirus from military dogs with diarrhea; Richmond: U.S. Animal Health Assoc.; 1975. pp. 359–366. [PubMed] [Google Scholar]
  • 6.Burtonboy G., Coignoul F., Delferriere N., Pastoret P. P. Canine hemorrhagic enteritis: detection of viral particles by electron microscopy. Arch. Virol. 1979;61:1–11. doi: 10.1007/BF01320586. [DOI] [PubMed] [Google Scholar]
  • 7.Caul E. O., Ashley C. R., Egglestone S. I. Recognition of human enteric coronaviruses by electron microscopy. Med. Lab. Sci. 1977;34:259–263. [PubMed] [Google Scholar]
  • 8.Caul E. O., Egglestone S. I. Further studies on human enteric coronaviruses. Arch. Virol. 1977;54:107–117. doi: 10.1007/BF01314383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 9.Cooper B. J., Carmichael L. E., Appel M. J. G., Greisen H. Canine viral enteritis. II. Morphologic lesions in naturally occurring parvovirus infection. Cornell Vet. 1979;69:134–144. [PubMed] [Google Scholar]
  • 10.Eugster A. K., Bendele R. A., Jones L. P. Parvovirus infection in dogs. J. Amer. vet. med. Ass. 1978;173:1340–1341. [PubMed] [Google Scholar]
  • 11.Flewett T. H., Bryden A. S., Davies H. A., Woode G. N., Bridger J. C., Derrick J. M. Relation between viruses from acute gastroenteritis of children and newborn calves. Lancet. 1974;ii:61–63. doi: 10.1016/s0140-6736(74)91631-6. [DOI] [PubMed] [Google Scholar]
  • 12.Flewett T. H., Bryden A. S., Davies H. A. Diagnostic electron microscopy of faeces. I. The viral flora of the faeces as seen by electron microscopy. J. clin. Pathol. 1974;27:603–608. doi: 10.1136/jcp.27.8.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 13.Hajer I., Storz J. Structural polypeptides of the enteropathogenic bovine coronavirus strain LY-138. Arch. Virol. 1979;59:47–57. doi: 10.1007/BF01317894. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 14.Keenan K. P., Jevis H. R., Marchwicki R. H., Binn L. N. Intestinal infection of neonatal dogs with canine coronavirus 1–71: studies by virologic, histologic, histochemical and immunofluorescent techniques. Amer. J. vet. Res. 1976;37:247. [PubMed] [Google Scholar]
  • 15.Kurtz J. B., Lee T. W., Pickering D. Astrovirus associated gastroenteritis in a children's ward. J. clin. Pathol. 1977;30:948–952. doi: 10.1136/jcp.30.10.948. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16.Maass G., Baumeister H. G., Hergemoller R., Jansen P., Schumacher H. Visualization by electronmicroscopy of 28 nm virusparticles (astroviruses) in faeces of newborns with acute nonbacterial gastroenteritis. Zbl. Bakt. Hyg., I. Abt. Orig. 1978;A242:423–430. [PubMed] [Google Scholar]
  • 17.McIntosh K. Coronaviruses: a comparative review. Curr. Top. Microbiol. Immunol. 1974;63:85–129. [Google Scholar]
  • 18.Madeley C. R., Cosgrove B. P. Viruses in infantile gastroenteritis. Lancet. 1975;ii:124. doi: 10.1016/s0140-6736(75)90020-3. [DOI] [PubMed] [Google Scholar]
  • 19.Madeley C. R., Cosgrove B. P. 28 nm particles in faeces in infantile gastroenteritis. Lancet. 1975;ii:451–452. doi: 10.1016/s0140-6736(75)90858-2. [DOI] [PubMed] [Google Scholar]
  • 20.Madeley C. R., Cosgrove B. P. Calicivirus in man. Lancet. 1976;i:199–200. doi: 10.1016/s0140-6736(76)91309-x. [DOI] [PubMed] [Google Scholar]
  • 21.Madeley C. R. Comparison of the features of astroviruses and caliciviruses seen in samples of feces by electron microscopy. J. inf. Dis. 1979;139:519–523. doi: 10.1093/infdis/139.5.519. [DOI] [PubMed] [Google Scholar]
  • 22.Mathan M., Mathan V. I., Swaminathan S. P., Yesudoss S., Baker S. J. Pleomorphic virus-like particles in human faeces. Lancet. 1975;i:1068–1069. doi: 10.1016/S0140-6736(75)91832-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 23.Middleton P. J., Szymanski M. T., Petric M. Viruses associated with acute gastroenteritis in young children. Amer. J. Dis. Child. 1977;131:733–737. doi: 10.1001/archpedi.1977.02120200015004. [DOI] [PubMed] [Google Scholar]
  • 24.Pocock D. H. Effect of sulphydryl reagents on the biological activities, polypeptide composition and morphology of haemagglutinating encephalomyelitis virus. J. gen. Virol. 1978;40:93–101. doi: 10.1099/0022-1317-40-1-93. [DOI] [PubMed] [Google Scholar]
  • 25.Pollock R. V. H., Carmichael L. E. Canine viral enteritis. Mod. Vet. Pract. 1979;60:375–380. [PubMed] [Google Scholar]
  • 26.Siegl G. The Parvoviruses. Wien-New York: Springer; 1976. p. 109. [Google Scholar]
  • 27.Snodgrass D. R., Gray E. W. Detection and transmission of 30 nm virus particles (astroviruses) in faeces of lambs with diarrhoea. Arch. Virol. 1977;55:287–291. doi: 10.1007/BF01315050. [DOI] [PubMed] [Google Scholar]
  • 28.Snodgrass D. R., Angus K. W., Gray E. W., Menzies J. D., Paul G. Pathogenesis of diarrhea caused by astrovirus infections in lambs. Arch. Virol. 1979;60:217–226. doi: 10.1007/BF01317493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 29.Spratt H. C., Marks M. I., Gomersall M., Gill P., Pai C. H. Nosocomial infantile gastroenteritis associated with minirotavirus and calicivirus. J. Pediatr. 1978;93:922–926. doi: 10.1016/S0022-3476(78)81212-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 30.Stair E. L., Rhodes M. B., White R. G., Mebus C. A. Neonatal calf diarrhea: purification and electron microscopy of a coronavirus-like agent. Amer. J. vet. Res. 1972;33:1147–1156. [PubMed] [Google Scholar]
  • 31.Tyrrell D. A. J., Alexander D. J., Almeida J. D., Cunningham C. H., Easterday B. C., Garwes D. J., Hierholzer J. C., Kapikian A., Macnaughton M. R., McIntosh K. Coronaviridae: Second report. Intervirology. 1978;10:321. doi: 10.1159/000148996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 32.Woode G. N., Bridger J. C. Isolation of small viruses resembling astroviruses and calciviruses from acute enteritis of calves. J. med. Microbiol. 1978;11:441–452. doi: 10.1099/00222615-11-4-441. [DOI] [PubMed] [Google Scholar]

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